Advancing respiratory–cardiovascular physiology with the working heart–brainstem preparation over 25 years

JFR Paton; BH Machado; DJA Moraes; DB Zoccal; AP Abdala; JC Smith; VR Antunes; D Murphy; M Dutschmann; RR Dhingra; R McAllen; AE Pickering; RJA Wilson; TA Day; NO Barioni; AM Allen; C Menuet; J Donnelly; I Felippe; WM St-John

Journal article

Advancing respiratory–cardiovascular physiology with the working heart–brainstem preparation over 25 years

JFR Paton, BH Machado, DJA Moraes, DB Zoccal, AP Abdala, JC Smith, VR Antunes, D Murphy, M Dutschmann, RR Dhingra, R McAllen, AE Pickering, RJA Wilson, TA Day, NO Barioni, AM Allen, C Menuet, J Donnelly, I Felippe, WM St-John

Journal of Physiology | Published : 2022

DOI: 10.1113/JP281953

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Abstract

Twenty-five years ago, a new physiological preparation called the working heart–brainstem preparation (WHBP) was introduced with the claim it would provide a new platform allowing studies not possible before in cardiovascular, neuroendocrine, autonomic and respiratory research. Herein, we review some of the progress made with the WHBP, some advantages and disadvantages along with potential future applications, and provide photographs and technical drawings of all the customised equipment used for the preparation. Using mice or rats, the WHBP is an in situ experimental model that is perfused via an extracorporeal circuit benefitting from unprecedented surgical access, mechanical stability of ..

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University of Melbourne Researchers

Robin McAllen Author

Andrew Allen Author

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Grants

Awarded by Neurological Foundation of New Zealand

Funding Acknowledgements

A.P.A. -R01 AT008632 -CRCNS. A.M.A. -Australian Research Council (DP170104582) and Australian National Health and Medical Research Council (APP1156727). V.R.A. Sao Paulo Research Foundation (FAPESP 2019/19894-8) and National Council for Development of Science and Technology (CNPq -Research Fellow: no. 304970/2017-4). R.R.D. -National Institutes of Health (U01 EB021960). J.D. -Clinical Fellowship from the Neurological Foundation of New Zealand. I.S.A.F. -University of Auckland PhD scholarship. B.H.M. Sao Paulo Research Foundation (FAPESP-2018/15957-2) and the National Council for Development of Science and Technology (CNPq-309338/2020-4). C.M. -Agence Nationale de la Recherche (ANR-21-CE14-0009-01). D.J.A.M. -FAPESP (2019/11863-6 and 2021/06886-7) and CNPq (437375/2018-8 and 313719/2020-9). J.F.R.P. -Health Research Council of New Zealand (19/687) and the Sidney Taylor Trust. A.E.P. was funded by a Wellcome Trust Clinical research fellowship. J.C.S. -Intramural Research Program of the NIH, NINDS. T.A.D. -Natural Sciences and Engineering Research Council of Canada Discovery grants (NSERC RGPIN-2016-04915). R.J.A. W. -Canada Institute of Health Research (CIHR201603PJT/366421). N.O.B. -SIDS Calgary Society.